Investigation of the `switch-epitope' concept with random peptide libraries displayed as thioredoxin loop fusions
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چکیده
منابع مشابه
Screening phage-displayed combinatorial peptide libraries.
Among the many techniques available to investigators interested in mapping protein-protein interactions is phage display. With a modest amount of effort, time, and cost, one can select peptide ligands to a wide array of targets from phage-display combinatorial peptide libraries. In this article, protocols and examples are provided to guide scientists who wish to identify peptide ligands to thei...
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mAb73, a monoclonal antibody against adenoviruses type 2 and 5 E1A, recognises an epitope within the C-terminal part of this protein. To identify the epitope we used random peptide libraries expressed on the surface of filamentous phages (Fd, M13). We found a consensus sequence homologous to the nuclear transport signal KRPRP at the C-terminus of Ad2 and Ad5 E1A. An E1A mutant deleted for these...
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Yoichi KUMADA, Masao NOGAMI, Fida HASAN, Masaaki TERASHIMA and Shigeo KATOH Department of Bioscience and Biotechnology, Okayama University, 1-1, Tsushimanaka 3, Okayam-shi, Okayama 700-8530, Japan Graduate School of Science and Technology, Kobe University, 1-1, Rokkodaicho, Kobe-shi, Hyogo 657-8502, Japan Graduate school of Human Sciences, Kobe College, 4-1, Okadayama, Nishinomiya-shi, Hyogo 66...
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Phage display techniques have been widely employed to map the epitope structures which served as the basis for developing molecular vaccines. In the present study, we applied this technique to map the epitopes of Mycoplasma hyopneumoniae, the etiologic agent causing swine enzootic pneumonia, and evaluated directly the immune responses in mice of the selected phage-displayed epitopes (phagotopes...
متن کاملIdentification and characterization of Src SH3 ligands from phage-displayed random peptide libraries.
We have used the Src homology 3 (SH3) domain to screen two phage-displayed random peptide libraries, each containing 2 x 10(8) unique members, and have identified a series of high affinity peptide ligands. The peptides possess similar proline-rich regions, which yield a consensus Src SH3-binding motif of RPLPPLP. We have confirmed this motif by screening a phage-displayed peptide library biased...
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ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2001
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/14.5.367